Technologies

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4th generation sequencing technique innovated by Genvida.

SONAS

(SOlid State NAnopore Sequencing)

SONAS makes use of proprietary technique to achieve massive and parallel fabrication of nanopore arrays with pore size down to <5 nm in one shot. Since the fabrication involves only semiconductor manufacturing compatible processes which are highly reproducibility with nanometer scale accuracy, the nanopore array fabrication can be easily scaled up for wafer-scale mass production.

Combining with the uniquely developed sample preparation technique (gPrep) and integrated bioinformatics suite, SONAS offers a drastic improvement over the competing technologies. Advantageously, Genvida’s direct read SONAS technology permits exceptionally high sensitivity at single molecule level rendering PCR or other types of amplification unnecessary, hence simplified workflows; moreover, uniquely because of the optimal combination of mean read length and unprecedentedly high throughput, SONAS makes the lowest possible per base and per run cost, and with the assurance of high quality results by our systems’ ultrahigh accuracy (>99.9% per base, i.e. an order of magnitude better comparing to the current best).

As a low cost, real time sequencing system with high accuracy, high throughput, fast sequencing speed and long read length is required to unlock the potential market, which entails a drastic change in the current sequencing approach and a quantum leap in sequencing technology. With the “all physical processes” approach adopted by SONAS, Genvida possesses a definite competitive edge to knock out current technologies. This will enable a much wider market than that of the current technologies. Data driven, informed decisions can be made by the physicians, the government officials and, ultimately, the patients themselves.

SONAS-2

Built upon a set of common core patented technologies, our platform patented technology SONAS can germinate into different families of high value, high growth products and applications, such as genome mapping (gMapper), digital PCR (dPCR), and single cell systems (sCell), which will be developed at a later stage for potential business expansion in the future.

Fundamentally, our SONAS based sequencing platform overhauls the underlying operating principle of the aforementioned ‘shotgun sequencing’ by offering an easy to use portable sequencing system, which includes gPrep: a simple sample preparation procedure/protocol, nSeq: a single-use disposable biochip, nanoSequencer: an integrated portable sequence analyzer, and bioSoft: an initiative, powerful bioinformatic software suite.

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Our Platform

gPrep

Instead of randomly cut the nucleic acids by ultrasound to create the short oligos for shotgun sequencing, we make use of the endonucleases to systematically cut the nucleic acids according to the restriction sites. The restriction sites will serve as markers and will greatly simplify the assembly and mapping of the reads during the post-detection analysis. Adaptable sample preparation protocols compatible to many NGS technologies.

nSeq

nSeq is a disposal biochip with full microfluidics and detection circuitry for nanopore sequencing. This biochip houses the automated sample delivery and an array of solid-state nanopores. In each nanopore, there are embedded electrodes, from which the physics based nucleotide detections are performed.

nanoSequencer

This portable unit serves as the command and control of the nSeq biochip, the interface between the nSeq biochip and the operator, and the interface between the remote server and the operator. It will store and analyze the data coming from the nSeq biochip, and will transmit the data and results to the remote server. It reports the current status of the sequencing process.

bioSoft

It is an integrated bioinformatics suite allowing rapid field diagnostics. Both the nanoSequencer and the remote server will have the software suite. They work in concert to analyze the data and to report the sequencing results.

gVault

With emerging sequencing results and analyses by SONAS and through collaboration with other universities, research institutes and hospitals, systematic cloud databases for bioinformation will be created for further exploitation in a variety of applications, e.g. personalized medicines, bio-screening, bio-surveillance, gene profiling, and etc.